Assessment and Quantification of Pharmacologically Active Marker Gallic Acid and Ellagic Acid from Leaf and Stem of Pergularia daemia Forsk. by HPTLC Method
A TLC densitometry method is developed for the quantification of gallic acid and ellagic acid using HPTLC. HPTLC is an important tool for determining chemical constituents in plant material. This is the first time that these two biologically active compounds, gallic acid and ellagic acid, have been quantified using HPTLC from this plant. Gallic acid and ellagic acid were quantified from the methanol extract using the Toluene: Ethyl acetate: Formic acid (6:4:0.8 v/v/v) solvent method. Gallic acid and ellagic acid have Rf values of 0.43 and 0.38, respectively. Gallic acid (100 to 600 ng) and ellagic acid (100 to 600 ng) have identical linearity levels, with correlation coefficients (r- values) of 0.9994 and 0.9997, respectively. Gallic acid and ellagic acid concentrations were 3.01 and 11.09 g/ml in the leaf and 4.97 and 18.30 g/ml in the stem, respectively. Gallic acid and ellagic acid quantification demonstrated strong separation and resolution from other extract constituents. The flexibility, precision, and selectivity of this approach are its key advantages. Its key benefits are its ease of use, precision, and selectivity. This approach can also be used to estimate these compounds in other herbal preparations, and it can help with standardisation.
Author (s) Details
Dr. N. G. Sutar
Sanjivani College of Pharmaceutical Education and Research, Kopargaon, Maharashtra, India.
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