News Update on Mannanase Research: Aug – 2019

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News Update on Mannanase Research: Aug – 2019

August 24, 2019 Biotechnology 0

Crystallization and Some Properties of Mannanase

A mannan-hydrolyzing accelerator created by _??_ bound strain of grass bacillus was pure from the culture broth and isolated in an exceedingly crystalline state by being treated with many ion-exchangers. The optimum pH scale of the accelerator was half dozen.0. The accelerator was stable in an exceedingly pH scale region of five.0 to 9.5 and at temperatures but five°C. The accelerator attacked solely β-1, 4-mannosidic linkages within the main chain of galactomannan of soybean testa, guar, gum and occasional bean, and of glucomannan of konjak (Amorphophalus konjac). [1]

Mannanase production by the lettuce endosperm

Endo-β-mannanase (EC three.2.1.78) is created and secreted by the cells of the reproductive structure of lettuce (lactuca sativa L.) “seeds” (achenes). In imbibed intact seeds, production is prevented by inhibitors. If the reproductive structure is incubated alone, these inhibitors may be removed by natural action, permitting mannanase production. Abscisic acid, a element of lettuce seeds, inhibits the assembly of mannanase within the isolated reproductive structure, and should be concerned in regulation of mannanase production in intact seeds. throughout germination the inhibition is removed, starting 4–8 h when red-light irradiation, that was given four h from sowing. [2]

A Germination-Specific Endo-β-Mannanase Gene Is Expressed in the Micropylar Endosperm Cap of Tomato Seeds

Endo-β-mannanase (EC three.2.1.78) is concerned in chemical reaction of the mannan-rich cell walls of the tomato (Lycopersicon esculentum Mill.) reproductive structure throughout germination and post-germinative spermatophyte growth. totally different cataphoretic isoforms of endo-β-mannanase ar expressed consecutive in numerous components of the reproductive structure, at first within the aperture reproductive structure cap covering the structure tip and later on within the remaining lateral reproductive structure encompassing the remainder of the embryo. we’ve isolated a complementary DNA from imbibed tomato seeds (LeMAN2) that shares seventy seven deduced aminoalkanoic acid sequence similarity with a post-germinative tomato mannanase (LeMAN1). [3]

High concentration and yield production of mannose from açaí (Euterpe oleracea Mart.) seeds via mannanase-catalyzed hydrolysis

The açaí seed corresponds to some eighty fifth of the fruit’s weight and represents ~1.1 million metric plenty of residue yearly accumulated within the Amazon region, leading to an acute environmental and concrete drawback. To extract the best price from this residue, this study aimed to guage its chemical composition to work out the suitable applications and to develop conversion strategies. First, mannan was confirmed because the major part of mature seeds, adore eightieth of the seed’s total carbohydrates and concerning five hundredth of its dry weight. [4]

Production of β-Mannanase by Penicillium italicum Subjected to Different Growth Conditions

Aims: the method parameters moving catalyst production were optimized to determine the simplest best conditions for β-mannanase production by fungus genus italicum in solid state fermentation.

Study Design: Four stages of experimental processes were designed for this study. the primary experiment, samples were withdrawn when twenty four, 48, 72, 96, 120, 144,168 and 192 h incubation.  In second experiment, the fermentation media were incubated at totally different temperatures. In third experiment, the result of various hydrogen ion concentration values on β-mannanase production was evaluated, whereas the fourth experiment delineate the supplementation of surfactants in mineral salt resolution for β-mannanase production. [5]

Reference

[1] Emi, S., Fukumoto, J. and Yamamoto, T., 1972. Crystallization and some properties of mannanase. Agricultural and Biological Chemistry, 36(6), pp.991-1001. (Web Link)

[2] Halmer, P. and Bewley, J.D., 1979. Mannanase production by the lettuce endosperm. Planta, 144(4), pp.333-340. (Web Link)

[3] Nonogaki, H., Gee, O.H. and Bradford, K.J., 2000. A germination-specific endo-β-mannanase gene is expressed in the micropylar endosperm cap of tomato seeds. Plant Physiology, 123(4), pp.1235-1246. (Web Link)

[4] High concentration and yield production of mannose from açaí (Euterpe oleracea Mart.) seeds via mannanase-catalyzed hydrolysis
Alvaro Ferreira Monteiro, Ingrid Santos Miguez, João Pedro R. Barros Silva & Ayla Sant’Ana da Silva
Scientific Reportsvolume 9, Article number: 10939 (2019) (Web Link)

[5] Olaniyi, O. O. and Akinyele, B. J. (2018) “Production of β-Mannanase by Penicillium italicum Subjected to Different Growth Conditions”, Biotechnology Journal International, 22(1), pp. 1-8. doi: 10.9734/BJI/2018/6763. (Web Link)

 

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