News Update on Thiobarbituric Acid Research: Aug – 2019

Science Press Release Distribution Services

News Update on Thiobarbituric Acid Research: Aug – 2019

August 31, 2019 Chemistry 0

The thiobarbituric acid assay of sialic acids

Publisher outline This chapter discusses the various aspects of thiobarbituric acid assay of sialic acid. Periodate oxidisation of the neuraminic acid backbone of sialic acids leads to the formation of β -formylpyruvic acid from carbon atoms one to four. The N -acetyl or N -glycolyl cluster of sialic acids apparently doesn’t interfere with periodate oxidisation. β -Formylpyruvic acid is as well as 2-thiobarbituric acid to create a red radical with a most absorption at 549 mμ. [1]

Coloring conditions of thiobarbituric acid test for detecting lipid hydroperoxides

The coloring reaction of the thiobarbituric appraisal for hydroperoxides was utterly inhibited  by the addition of EDTA. Therefore, it had been necessary to feature a metal salt to the reaction mixture to finish the reaction associated additionally to add an inhibitor to forestall autoxidation once unoxidized unsaturated fatty acids co‐exist. The optimum pH scale of the reaction was found at three.6 victimisation glycine‐hydrochloric acid buffer. [2]

Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds

The incidence of malondialdehyde (MDA), a secondary end result of the oxidisation of unsaturated fatty acids, is taken into account a helpful index of general macromolecule peroxidation. a typical methodology for measurement MDA, brought up because the thiobarbituric acid-reactive-substances (TBARS) assay, is to react it with thiobarbituric acid (TBA) and record the absorbance at 532 nm. However, several plants contain busybodied compounds that conjointly absorb at 532 nm, resulting in overestimation of MDA values. Extracts of plant tissues together with purple eggplant (Solanum melongena L.) fruit, carrot (Daucuscarota L.) roots, and spinach (Spinacia oleracea L.) leaves were assessed for the presence of MDA and alternative non-MDA compounds gripping at 532 nm. [3]

Estimation of 3-Deoxy Sugars by Means of the Malonaldehyde–Thiobarbituric Acid Reaction

DURING the course of our investigations on the biogenesis of three,6-dideoxy-hexoses, found within the endotoxins of enterics and enterics 1–3 and in the eggs of Parascaris equorum four, the necessity arose for a sensitive and specific assay. A thought of the structures of those compounds urged to United States that the malonaldehyde–thiobarbituric acid reaction, that was tailored by Waravdekar and Saslaw5 for the estimation of 2-deoxy-ribose, may equally be applicable to the quantitative determination of three,6-dideoxy-hexoses, and indeed, of 3-deoxy sugars normally. [4]

Green Chemistry Approach for Synthesis of Bioactive 2-Thiobarbituric Acid Derivatives

Aims: the subsequent study is aimed to organize 5 derivatives of 2-thiobarbituric acid below microwave irradiation (MWI) and standard heating methodology and investigate their biological activities.

Study Design: A classic white aminoalkanoic acid Microwave (720 W, 2450 MHz) with 9 power settings was used for this study.

Place and period of Study: Department of Applied Chemistry & Chemical Engineering, Islamic University, Kushtia, Bangladesh, between June 2012 and Gregorian calendar month 2014. [5]

Reference

[1] Warren, L., 1959. The thiobarbituric acid assay of sialic acids. J biol chem, 234(8), pp.1971-1975. (Web Link)

[2] Asakawa, T. and Matsushita, S., 1980. Coloring conditions of thiobarbituric acid test for detecting lipid hydroperoxides. Lipids, 15(3), pp.137-140. (Web Link)

[3] Hodges, D.M., DeLong, J.M., Forney, C.F. and Prange, R.K., 1999. Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta, 207(4), pp.604-611. (Web Link)

[4] Estimation of 3-Deoxy Sugars by Means of the Malonaldehyde–Thiobarbituric Acid Reaction
MORRIS A. CYNKIN & GILBERT ASHWELL
Naturevolume 186, pages155–156 (1960) (Web Link)

[5] Khatun, K., A. Sattar, M., Nahar, K. and Alauddin, J. (2018) “Green Chemistry Approach for Synthesis of Bioactive 2-Thiobarbituric Acid Derivatives”, Asian Journal of Physical and Chemical Sciences, 5(3), pp. 1-7. doi: 10.9734/AJOPACS/2018/40562. (Web Link)

 

Leave a Reply

Your email address will not be published. Required fields are marked *