News Update on Tissue Culture Research: Aug – 2019

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News Update on Tissue Culture Research: Aug – 2019

August 23, 2019 Biotechnology 0

Plant propagation by tissue culture. Part 1: the technology

First printed in 1984, this can be wide used as a book of facts for laboratories and researchers. The 2d edition is totally revised and greatly extended and is therefore showing in two halfs; the 2d part, particularisation the follow of propagating plants in vitro, is being printed approx. one year when the first half. half one deals with the technology of micropropagation within the following chapters: plant structure culture techniques; plant propagation and micropropagation; variation in cultures and regenerated plants; instrumentality and procedures; dominant persistent contaminants and plant diseases; storing and distributing being material; factors touching growth and ontogeny (genotype and therefore the physical setting, and tissue-dependent factors); the elements of culture media; the derivation, preparation and use of plant culture media; and plant growth regulators. The text is illustrated with figures and plates. [1]

Differentiated Rat Glial Cell Strain in Tissue Culture

Rat interstitial tissue tumors, induced  by injections of N-nitrosomethylurea, were plated and propagated in culture. Among some cell strains obtained, one clone contains S-100 macromolecule, that is exclusive to brain in vertebrates. Stationary-phase cultures contain just about 10 times a lot of S-100 macromolecule per cell than exponentially growing cells. once injected into newborn rats, cells manufacturing S-100 grew as a interstitial tissue tumour, that contained S-100 macromolecule. [2]

Derivation of specific antibody‐producing tissue culture and tumor lines by cell fusion

Cell fusion techniques are accustomed turn out hybrids between malignant neoplasm cells and antibody‐producing cells. The hybrid lines derived are for good custom-made to grow in tissue culture and are capable of causing antibody‐producing tumors in mice.

Spleens from mice insusceptible against sheep red blood cells (SRBC) were amalgamated to Associate in Nursing 8‐azaguanine‐resistant clone (X63‐Ag8) of MOPC twenty one malignant neoplasm. Over fifty you look after the derived hybrid lines turn out and secrete immunoglobulins totally different from the MOPC twenty one malignant neoplasm. concerning ten you look after the hybrid lines exhibit anti‐SRBC activity. The high proportion of antibody‐producing hybrids suggests that the fusion involves a restricted fraction of the spleen cell population, in all probability cells committed to protein production. [3]

Long-term functional and structural preservation of precision-cut human myocardium under continuous electromechanical stimulation in vitro

In vitro models incorporating the quality and performance of adult human tissues are extremely desired for translational  analysis. while very important slices of human heart muscle approach these demands, their speedy degeneration in tissue culture precludes long experimentation. Here, we tend to report preservation of structure and performance of human heart muscle below conditions of physiological preload, compliance, and continuous excitation. In biomimetic culture, tissue slices ready from explanted failing human hearts attain a stable state of ability that may be monitored for up to four months or 2000000 beats in vitro. [4]

Use of Agro-Wastes for Tissue Culture Process and Spawn Production of Oyster Mushroom (Pleurotus florida)

This study investigated the potentials of various growth media for the tissue culture method of oyster fungus moreover as evaluated the response of different agro-wastes in oyster fungus spawn production. Agro-waste Powder Agar was ready by admixture fine agro-wastes that are corncobs and sugarcane pulp, with agar within the magnitude relation 10:15. Twenty-five grams every of the media was suspended in 500ml of H2O and autoclaved suitably. Potato grape sugar Agar (PDA) was used as management growth medium. Oyster mushroom’s plant part was surface sterilized, drained and transferred unto the expansion media in an exceedingly streamline flow. [5]

Reference

[1] George, E.F., 1993. Plant propagation by tissue culture. Part 1: The technology (No. Ed. 2). Exegetics limited. (Web Link)

[2] Benda, P., Lightbody, J., Sato, G., Levine, L. and Sweet, W., 1968. Differentiated rat glial cell strain in tissue culture. Science, 161(3839), pp.370-371. (Web Link)

[3] Köhler, G. and Milstein, C., 1976. Derivation of specific antibody‐producing tissue culture and tumor lines by cell fusion. European journal of immunology, 6(7), pp.511-519. (Web Link)

[4] Long-term functional and structural preservation of precision-cut human myocardium under continuous electromechanical stimulation in vitro
Carola Fischer, Hendrik Milting, Evelyn Fein, Elisabeth Reiser, Kun Lu, Thomas Seidel, Camilla Schinner, Thomas Schwarzmayr, Rene Schramm, Roland Tomasi, Britta Husse, Xiaochun Cao-Ehlker, Ulrich Pohl & Andreas Dendorfer
Nature Communicationsvolume 10, Article number: 117 (2019) (Web Link)

[5] Ayobami Bankole, F. and Olusola Salami, A. (2017) “Use of Agro-Wastes for Tissue Culture Process and Spawn Production of Oyster Mushroom (Pleurotus florida)”, Journal of Applied Life Sciences International, 14(1), pp. 1-9. doi: 10.9734/JALSI/2017/35858. (Web Link)

 

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