β-Lactam Antibiotic Induces the Expression of blaOXA-51-like and blaOXA-23 in Clinically Isolated Acinetobacter baumannii and Acinetobacter lowffii
Acinetobacter is globally responsible for nosocomial infections as opportunistic pathogens. It is known for its ability to withstand a variety of antibiotics and belongs to gamma protoebacteria, Gram-negative, aerobic bacteria. Two isolates of A. In this study, Bassaffii, and A. 16S rRNA assessment with a high percentage similarity to the corresponding strains on the NCBI server, with accession numbers MH685113 and MH685112 for A, were collected clinically and genomically identified. Baumannii, A. Baseffii, respectively. The isolates’ sensitivity profile was variable, and A. In contrast with A, baumannii was the most resistant strain to a wide variety of antibiotics and displayed no growth defects in the presence of β-lactam antibiotics. Bassoffii. We found that imipenem resistance in A was responsible for the blaOXA-23 variant gene. Whereas baumannii, blaOXA-51-like was moderately immune to A. Lowffii, absence of blaOXA-23. This was measured when the isolates were exposed to qRT-PCR. We observed that in the presence of imipenem, the blaOXA-23 variant gene increased around 1-fold, while in comparison to the regulation, blaOXA-51-like did not increase. Bioinformatic studies have shown that blaOXA-23 is in the cytoplasm, and blaOXA-like 51 is in the periplasm, and this trend can lead to the outbreak of Acinetobacter multidrug resistance.
Halah H. Al-Haideri
Department of Biology, College of Science for Women, University of Baghdad, Baghdad 10072, Iraq.
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