A Comparative Study of in vivo Plant and in vitro Callus Extracts of Coccinia indica (W. and A)

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A Comparative Study of in vivo Plant and in vitro Callus Extracts of Coccinia indica (W. and A)

November 6, 2020 Biotechnology 0

An attempt was made in the present study to evaluate the phytochemical, antimicrobial , antioxidant and alpha-amylase inhibitory activity of leaf extracts of Coccinia indica (W. and A) using four solvents and to compare them with callous extracts. The leaf explants of C. were initiated by Callus. Indica with 90 % efficiency using BAP (1 mg / l) + NAA (0.2 mg / l) supplemented with MS medium. Successive C method of extraction. Indica was found to be an effective extraction method, and methanol was found to be the most suitable solvent for the extraction of phytochemicals and macromolecules responsible for inhibition of antimicrobials, antioxidants and alpha-amylase. Analysis by GC-MS of C. Indica has confirmed the presence of bioactive compounds (e.g. 9-octadecanoic acid, 2-octadecycloxy ethyl ester (100%) in successive methanolic callus extract) in all extracts where the presence of different important functional groups of the identified bioactive compounds has been confirmed by FTIR analysis. Successive extract of methanol from the callus of C. Indica was found to be a potent antimicrobial agent with drug efflux pump inhibitor properties against 5 strains of bacteria, Klebsiella pneumoniae (ATCC700603), Escherichia coli (ATCC25922), Staphylococcus aureus (ATCC 25923), Proteus mirabilis (ATCC 25933) and Pseudomonas aeruginosa (clinical isolate) and 3 strains of fungi, Candida albicans (IFM 40009), Candida tropicalis (IFM 55058) and Candida kruginosa (clinical isolate) Successive extract of methanol from the callus of C. Indica has been found to be an powerful antioxidant and alpha-amylase inhibitor, showing that it is a potent anti-diabetic agent with an IC50 concentration of 82.5μg / ml. One of the strong evidence for this plant to be used as a phytopharmaceutical agent by conventional practitioners is this research.

Author(s) Details

Dr. J. Anbumalarmathi
Department of Biotechnology, Stella Maris College (Autonomous), Chennai 600 086, India.

Dr. S. Aruna Sharmili
Department of Biotechnology, Stella Maris College (Autonomous), Chennai 600 086, India.

V. Jayalakshmi
Department of Biotechnology, Stella Maris College (Autonomous), Chennai 600 086, India.

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