Advanced Study on Antioxidant Properties of Silver Nanoparticles Biosynthesized from Methanolic Leaf Extract of Blighia sapida

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Advanced Study on Antioxidant Properties of Silver Nanoparticles Biosynthesized from Methanolic Leaf Extract of Blighia sapida

July 2, 2021 Engineering 0

The use of microorganisms, enzymes, or plant extracts in the synthesis of nanoparticles has been proposed as an ecofriendly alternative to chemical and physical methods that involve the use of harmful reducing agents. Silver nanoparticles (AgNPs) were synthesized using a green approach from methanolic leaf extract of Blighia sapida in the current study. UV-visible (UV-vis) spectroscopy, Fourier Transform Infrared (FTIR) spectroscopy, and Scanning Electron Microscopy were used to characterize the synthesized AgNPs (SEM). The antioxidant activity of the synthesized AgNPs was assessed using the DPPH radical scavenging assay, total reductive potential, total phenolics content (TPC), and total flavonoids content (TFC). The size of the synthesized silver nanoparticles ranged from 50 to 70 nm, with maximum UV-vis absorbance at 413 nm, according to SEM analysis. Radical scavenging activity of DPPH, reducing power, total phenolic and total flavonoid content When compared to ascorbic acid, the standard reference, the content of the synthesized AgNPs increased in a dose-dependent manner. This finding confirmed that Blighia sapida is a viable biomaterial for the production of AgNPs, which can then be used to boost antioxidant activity. This study sheds light on the use of Blighia sapida leaf as a good source of naturally occurring antioxidants, which could be useful as a therapeutic agent in the prevention or treatment of oxidative stress-related diseases.

Author (S) Details

Dr. A. O. Akintola
Department of Science Laboratory Technology, Faculty of Pure and Applied Sciences, Ogbomoso, Ladoke Akintola University of Technology, Nigeria.

Mr. B. D. Kehinde
Department of Biochemistry, Faculty of Basic Medical Sciences, Ladoke Akintola University of Technology, Ogbomoso, Nigeria.

Dr. P. B. Ayoola
Department of Science Laboratory Technology, Faculty of Pure and Applied Sciences, Ogbomoso, Ladoke Akintola University of Technology, Nigeria.

Dr. A. G. Adewoyin
Department of Science Laboratory Technology, Faculty of Pure and Applied Sciences, Ogbomoso, Ladoke Akintola University of Technology, Nigeria.

O. T. Adedosu
Department of Biochemistry, Faculty of Basic Medical Sciences, Ladoke Akintola University of Technology, Ogbomoso, Nigeria.

J. F. Ajayi
Department of Physiology, Faculty of Basic Medical Sciences, Ladoke Akintola University of Technology, Ogbomoso, Nigeria.

Mr. S. B. Ogunsona
Department of Science Laboratory Technology, Faculty of Pure and Applied Sciences, Ogbomoso, Ladoke Akintola University of Technology, Nigeria.

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