Analysing Activity of Cellulase and Macerating Enzyme and Effect of Catechin on Cellulase Activity
Cellulose having linkages like β-1,4-linked homopolymer of glucose is major and primary portion in plant cell wall. Detection of released monosaccharides after enzyme treatment with suitable required cell ambience can unleash the detailed mechanism of optimum activity of cellulase and macerating enzyme. Efficiency of enzyme effect can be studied on sample plant particles. Cellulases and macerating enzyme hydrolyse cellulose into monosaccharides like glucose, dextrose and fructose. Cellulase activity may be affect by presence of catechin, thus catechin effect can be either inhibiting or facilitating the reaction. The detailed mechanism is discussed in the article.
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Plant Biotechnology Laboratory, Department of Botany, Ramniranjan Jhunjhunwala College, Ghatkopar (West), Mumbai – 400086 (M.S.), India.
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