Assessment of Antibacterial Susceptibility Profiles of Gongronema latifolium Extracts and Alum against Clinical Bacterial Pathogens

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Assessment of Antibacterial Susceptibility Profiles of Gongronema latifolium Extracts and Alum against Clinical Bacterial Pathogens

October 9, 2020 Biotechnology and Microbiology 0

The antimicrobial susceptibility test for Gongronema latifolium extracts in combination with potash alum (Alum) was tested using disc diffusion (DD) and Agar well diffusion (AWD) protocols against some clinical bacterial pathogens. Until application, ethanolic and methanolic leaf extracts at concentrations of 0.1-0.3 g were reconstituted in sterile distilled water and 1.0-3.0 g of potash alum. In vitro bioactivity of different extract concentrations and, in combination with alum, measurements of the diameter of inhibition zones (DIZs) were evaluated. The highest inhibition zones for Escherichia coli (14.5±0.5) and Salmonella typhi (11.5±0.0 mm) with enhanced bioactivity of 19.5±0.7 and 17.5±0.7 mm with potash alum against Bacillus subtilis, Sal. were obtained with methanolic leaf extract (MLE). By DD and AWD processes, respectively, typhi and Pseudomonas aeruginosa. Aqueous leaf extract (ALE) and ethanolic leaf extract (ELE), however, and their extracts Combinations of the pathogens displayed strong antibacterial activity but were incomparable to MLE. Bioactivity improvement was achieved with the addition of Alum to leaf extracts on a dose response fashion, particularly by the AWD process, irrespective of the solvent of extraction. In addition , the low MIC values of < 0.05 to 0.2mg / ml for MLE and ALE bacteria confirm their potency and wide spectrum activity. Ciprofloxacin (CP)’s very large DIZs, on the other hand, reflect the beneficial effect of distilled chemotherapeutics against pathogens. The increased efficacy of these natural material extracts, such as potash alum, would also justify potential applications in ethnomedicine, nutraceuticals, pharmaceuticals and food systems.

Author (s) Details

Lawrence O. Amadi
Department of Microbiology, Faculty of Science, Rivers State University, P.M.B. 5080, Nkpolu-Oroworukwo, Port Harcourt, Nigeria and Department of Microbiology, School of Applied Science, Ken Saro-Wiwa Polytechnic, P.M.B. 20, Bori, Rivers State, Nigeria.

Nathaniel N. Ngerebara
Department of Microbiology, School of Applied Science, Ken Saro-Wiwa Polytechnic, P.M.B. 20, Bori, Rivers State, Nigeria.

Chidinma A. Okafor
Department of Microbiology, School of Applied Science, Ken Saro-Wiwa Polytechnic, P.M.B. 20, Bori, Rivers State, Nigeria.

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