Description of a Harmless Method for Determining Trenbolone Acetate Together with 17β-Trenbolone in Beef
The simultaneous detection of trenbolone acetate and 17-trenbolone in cattle muscle is described using a cost-effective, safe, and quick sample preparation method followed by reversed-phase high-performance liquid chromatography (HPLC). On a short C1 column with an isocratic mobile phase, HPLC analysis with a photo-diode array detector was performed. Analyses of spiked samples, resulting recoveries (87.9%; relative standard deviations 3.4%), analytical total time (20 min/sample, where a batch of 12 samples was finished in 2 hours), and quantitation limits (1.8 ng/g) were used to verify the method. The proposed method for detecting TBa and -TB in beef (cattle muscle) is a valuable tool for routine residue monitoring in beef as well as beef farm withdrawal control. There were no hazardous organic solvents or reagents employed at all.
Author (s) Details
Graduate School of Human Life Science, Osaka City University, Osaka 558-8585, Japan.
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