Effect of Solvent on Catalysis of Protease Adsorbed on Biochar in Organic Media
The adsorption immobilization of the serine protease α-chymotrypsin (α-CT) onto bamboo charcoal powder (BCP), which is a kind of biochar, improved the transesterification rate of N-acetyl-L-tyrosine ethyl ester (N-Ac-Tyr-OEt) with n-butanol (BuOH) in 9 organic solvents. Organic solvents strongly affected the catalysis of BCP-adsorbed α-CT. The transesterification rate of BCP-adsorbed α-CT was much superior to that of free α-CT in every organic solvent. Especially, the transesterification rate of BCP-adsorbed α-CT was about 760 times higher than that of free α-CT in n-butyl acetate.
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Department of Applied Chemistry for Environment, Tokyo Metropolitan University, Tokyo, 192-0397, Japan.
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