Evaluation of Toxicity on Mice and Artemicidal Activity of Bauhinia monandra Leaf Lectin (BmoLL)
Aims: This work aimed to determine the toxicity of Bauhinia monandra leaf lectin (BmoLL) to mice and Artemia salina.
Study design: BmoLL was purified from the leaves of B. monandra through affinity chromatography and tested against mice and A. salina in order to evaluate its toxicity.
Methodology: The lectin purification was performed through saline precipitation and chromatography onto a guar gel column. The acute toxicity of BmoLL was evaluated using female mice during 14 days. Toxicity of BmoLL to A. salina larvae was also evaluated.
Results: BmoLL did not induce death or weight loss of mice at 2000 mg/kg b.w.; additionally, the lectin did not affect the survival of A. salina at the assayed concentrations (250–1,000 µg/mL).
Conclusion: BmoLL is an interesting molecule to investigate pharmacological applications due to its low toxicity according to the two models used in this work.
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Luana Cassandra Breitenbach Barroso Coelho
Departamento de Enfermagem, Faculdade dos Palmares – FAP, Palmares, Pernambuco, CEP 55540-000, Brazil.
Colegiado de Ciências Farmacêuticas, Universidade Federal do Vale do São Francisco – UNIVASF, Petrolina, Pernambuco, CEP 56304-917, Brazil.
Departamento de Bioquímica – CCB, Universidade Federal de Pernambuco – UFPE, Cidade Universitária, Recife, Pernambuco, CEP 50670-420, Brazil.
Centro Acadêmico do Agreste, Universidade Federal de Pernambuco – UFPE, Caruaru, Pernambuco, CEP 50670-901, Brazil.
Departamento de Medicina, Universidade de Pernambuco – UPE, Garanhuns, Pernambuco, CEP 55295-110, Brazil.