Improvement of Antioxidant Enzyme Level and Anti-Cadmium Activity in Cell-Lines Model as Effect of Thunbergia laurifolia Leaf Extract

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Improvement of Antioxidant Enzyme Level and Anti-Cadmium Activity in Cell-Lines Model as Effect of Thunbergia laurifolia Leaf Extract

June 23, 2022 Medical Science 0

Thunbergia laurifolia, also known as Rang Jued, has been utilised as a cleansing herb and in traditional medicine. Worldwide, there is a significant public health issue with cd pollution. Using cell models such as murine macrophage (RAW264.7), human embryonic kidney (HEK293), and human hepatocellular carcinoma (HepG2) as a tested cell model, it was hoped to determine the endogenous antioxidant enzyme activities and malondialdehyde (MDA) production of the crude dried extract of T. laurifolia leaves. Additionally, all cells’ cytotoxicity, including anti-cadmium (Cd) activity, was assessed. In order to investigate the anti-Cd toxicity, the experimental design was separated into three groups with combined, pre-, and post-treatments. Cell viability was assessed using the MTT test (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide). The malondialdehyde (MDA) levels in HEK293 and HepG2 cells were reduced by the crude extract, which also boosted catalase (CAT) and glutathione peroxidase (GPx) activities. When CaNa2EDTA, a chelating agent, was tried, the combination treatment effect was also discovered. Therefore, it was determined that the crude extract can shield cells from oxidative damage brought on by Cd. Additionally, mixing a chelating agent with the crude extract or drinking herbal tea should be more effective than utilising only the chelating agent or crude extract alone.

Author(s) Details:

Marasri Junsi,
Culinary Arts and Kitchen Management, Faculty of Hospitality Industry, Dusit Thani College, Pravet, Bangkok 10250, Thailand.

Sunisa Siripongvutikorn,
Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand.

Please see the link here: https://stm.bookpi.org/NHMMR-V11/article/view/7146

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