In vitro Generation of Human Adipose Derived Mesenchymal Stromal Cells Using α –MEM and DMEM

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In vitro Generation of Human Adipose Derived Mesenchymal Stromal Cells Using α –MEM and DMEM

July 7, 2021 Medicine and Medical Science 0

MEM and DMEM are the basic media for cell culture. When compared to DMEM, -MEM has a lower and more optimal concentration of glucose and amino acids. In this study, we compared the effects of these two media on the quantity, viability, and immunophenotyping of AD-MSC. The adipose tissue was extracted from the anterior abdominal wall of healthy, willing donors. It was divided into two tissue culture flasks with culture medium, one with -MEM and the other with DMEM. After incubating with collagenase-I, the pellet was resuspended in centrifuge tubes with phosphate buffered saline and divided into six equal parts. In 6-well plates, each set was incubated separately for in-vitro generation. The media was replenished on alternate days, with no passaging. Every third day, one well from each set was harvested using trypsinization. Flow cytometry was used to assess sterility, quantity, viability, and immunophenotyping (CD45-/90+/73+). We compared the maximum total cell count, viability, CD45-CD90+, and CD45-CD73+ population in -MEM to that in DMEM.

Author (S) Details

Dr. Aruna V. Vanikar
Department of Pathology, Laboratory Medicine, Transfusion Services and Immunohematology, G.R. Doshi and K.M. Mehta Institute of Kidney Diseases & Research Centre (IKDRC), Dr. H.L. Trivedi Institute of Transplantation Sciences (ITS), Civil Hospital Campus, Asarwa, Ahmedabad 380016, Gujarat, India and Department of Regenerative Medicine and Stem Cell Therapy, G.R. Doshi and K.M. Mehta Institute of Kidney Diseases & Research Centre (IKDRC), Dr. H.L. Trivedi Institute of
Transplantation Sciences (ITS), Civil Hospital Campus, Asarwa, Ahmedabad 380016, Gujarat, India.

Dr. Chetan N. Patel
Department of Pathology, Laboratory Medicine, Transfusion Services and Immunohematology, G.R. Doshi and K.M. Mehta Institute of Kidney Diseases & Research Centre (IKDRC), Dr. H.L. Trivedi Institute of Transplantation Sciences (ITS), Civil Hospital Campus, Asarwa, Ahmedabad 380016, Gujarat, India.

Dr. Kunal S. Gupte
Department of Pathology, Laboratory Medicine, Transfusion Services and Immunohematology, G.R. Doshi and K.M. Mehta Institute of Kidney Diseases & Research Centre (IKDRC), Dr. H.L. Trivedi Institute of Transplantation Sciences (ITS), Civil Hospital Campus, Asarwa, Ahmedabad 380016, Gujarat, India.

Umang G. Thakkar
Department of Regenerative Medicine and Stem Cell Therapy, G.R. Doshi and K.M. Mehta Institute of Kidney Diseases & Research Centre (IKDRC), Dr. H.L. Trivedi Institute of Transplantation Sciences (ITS), Civil Hospital Campus, Asarwa, Ahmedabad 380016, Gujarat, India.

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