Investigating the Sequence Variation in E2-Binding Domain of HPV16 and Biological Function Evaluation in Tunisian Cervical Cancers

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Investigating the Sequence Variation in E2-Binding Domain of HPV16 and Biological Function Evaluation in Tunisian Cervical Cancers

July 6, 2021 Medicine and Medical 0

Infection with hr-HPV (high-risk human papillomaviruses) is a necessary but insufficient cause of invasive cervical cancer, with additional virus-host interactions required for cancer to develop. The transcriptional activity of the LCR is affected differently by HPV16 E2 variants. The nucleotide and amino acid sequence variation within the HPV16 E2 gene was investigated in this study to see if it correlated with disease progression. The disruption of the E2 gene was discovered through PCR amplification of the entire E2 gene with a single set of primers. Bidirectional sequencing was used to examine nucleotide variations. A reverse transcriptase was used to examine the mRNA expression patterns of E6 and E7 gene transcripts. Controls outperformed cases (81.8 percent vs. 37.5 percent, p = 0.05). Variation at position 3684 C>A results in the amino acid substitution T310K in the E subgroup and was more common in the E2 undisrupted cases (7/9; 77.7 percent ) compared to controls (2/9; 22.2 percent ). Furthermore, specific sequence variations in the E2 ORF at positions 3684 C>A were linked to increased viral oncogene E6-E7 production. Aside from HPV16 E2 disruption, the 3684 C>A variation within undamaged E2 genes could be involved in another mechanism for deregulating expression. HPV16 E6 and E7 oncogenes and appears to be a major contributor to the development of cervical cancer in Tunisian women. The study would provide valuable information because the level of HPV16 E6-E7 transcripts and the detection of E2 mutations, combined with DNA physical state detection, could serve as an additional mechanism for assessing risk of cervical carcinoma development. of the HPV16 E6 and E7 oncogenes and appears to be a major factor contributing to the development of cervical cancer in Tunisian women. The study would provide valuable information because the level of HPV16 E6-E7 transcripts and the detection of E2 mutations, combined with DNA physical state detection, could serve as an additional mechanism for assessing risk for the development of cervical carcinoma.

Author (S) Details

Saloua Kahla
Unit of Immuno-Microbio-Environmental and Carcinogenesis (IMEC), Faculty of Sciences, University of Carthage, Jarzouna, 7021 Bizerta, Tunisia.

Lotfi Kochbati
Radio-oncology Department, Salah Azaiz Institute, 1006 Tunis, Tunisia.

Samia Hammami
Research Laboratory of Antimicrobial Resistance, Faculty of Medicine of Tunis, 1007 Tunis, Tunisia.

Prof. Mohamed Badis Chanoufi
Service of Gynaecology Obstetrics A, Center of Maternity and Neonatology, Hospital La Rabta, 1007 Tunis, Tunisia.

Mongi Maalej
Radio-oncology Department, Salah Azaiz Institute, 1006 Tunis, Tunisia.

Ridha Oueslati
Unit of Immuno-Microbio-Environmental and Carcinogenesis (IMEC), Faculty of Sciences, University of Carthage, Jarzouna, 7021 Bizerta, Tunisia.

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