Isolation, Characterizations of Bioactive Principles from Melia dubia Leaf and Its In-silico and In-vitro Urolithiatic Activity

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Isolation, Characterizations of Bioactive Principles from Melia dubia Leaf and Its In-silico and In-vitro Urolithiatic Activity

June 19, 2021 Chemical Science 0

Many medicinal plants contain a wide range of bioactive compounds at various levels. The primary goal of this research is to identify and separate antioxidants from different extracts of Melia dubia leaves. Soxhlet extraction was used with solvents such as hexane, chloroform, ethyl acetate, methanol, water, and 70% ethanol. The anti-oxidant capacity of six extracts was determined using the simple and convenient DPPH method. Extracts with high scavenging activity Separation was achieved using chromatographic techniques, which were then characterized using spectral methods. In-silico and in-vitro testing were performed on compounds with the highest antioxidant efficacy to determine their inhibitory potential against Urolithiatic activity. Methanol and ethanol have antioxidant activity. The antioxidant potential of 70% ethanol extracts was higher, and two antioxidants, MDL-1 and MDL-2, were isolated and characterized. MDL-1 had a higher scavenging potential than MDL-2, and its antiurolithiatic properties were confirmed using molecular docking (Insilico) and the single diffusion gel method (In-vitro).

Author (S) Details

Dr. J. Rosaline Vimala
PG & Research Department of Chemistry, Holy Cross College (Autonomous), Tiruchirappalli, India.

Dr. G. Dayana Jeyaleela
PG & Research Department of Chemistry, Holy Cross College (Autonomous), Tiruchirappalli, India.

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