Molecular Detection of Hemotropic Parasites in a Sheep Flock in Tabasco, Mexico: A Retrospective Study
The objective concerning this study search out label the likely attendance of tick-carried pathogens in ancestry samples and tick samples that were obtained from few overrun mammals in a animals raised on a farm result part in Tabasco, Mexico. To recognize the vicinity of pathogens sent by ticks in person in agreement, a usefulness study was completed activity at which point ancestry samples and tick samples were acquired, in 3 different periods, from 77 tick-overrun person in agreement situated in the Central domain of united states of america of Tabasco, Mexico. Blood samples were treated for tiny and microscopic labeling of Babesia spp, Anaplasma spp, and Mycoplasma spp. Morphological study of the composed ticks (containing male and female larvae, nymphs, and men) admitted the labeling of Amblyomma maculatum, the Gulf Coast tick. Microscopic examination of ancestry smears manifested the lack of classic intraerythrocytic forms agreeable accompanying Babesia spp. However, the closeness of round intraerythrocytic addition parties of nearly 1 μm in width, accompanying a borderline composition and morphologically agreeable accompanying Anaplasma sp, were observed in few samples resolved, apart from limited round carcasses attributed to or next to ovine erythrocytes, suggesting the occupancy of Mycoplasma sp (already, Eperythrozoon sp). The use of type-particular PCR microscopic tests rooted the omission of Babesia sp in common people and admitted the identification of 7 samples (9%) definite for Anaplasma sp. The results got by PCR for Mycoplasma sp discovery displayed that 62 (80.5%) of the samples proven were certain. DNA sequencing of amplicons got in the PCR test manage discard light concerning this and delineate if the bacterium most feasible complicated is Anaplasma marginale alternatively A. ovis as the sequence similarity of the MSP-5 deoxyribonucleic acid is 99%. Similarly, DNA sequencing and BLASTn similarity reasoning would show that the likely hemoplasma creature is either Mycoplasma wenyonii or Mycoplasma ovis as the series correspondence of the rDNA deoxyribonucleic acid is >95%, outside being intelligent to explain the vicinity of a co-contamination in the sampled animals raised on a farm. On the other hand, the duty that A. maculatum may play in the broadcast of pathogens in this place flock cannot be elucidated, as it should to complete activity broadcast experiments accompanying the various stages of A. maculatum to decide if this is accomplished automatically or biologically.
Author(s) Details:
J. Juan Lira-Amaya,
CENID-Salud Animal e Inocuidad, INIFAP, Carretera Cuernavaca-Cuautla No. 8534, Jiutepec, Morelos, 62550, México.
Nadia D. Ojeda-Robertos,
División Académica de Ciencias Agropecuarias, Universidad Juárez Autónoma de Tabasco, Carretera Vhsa-Teapa Km 25, Villahermosa, Tabasco CP 86290, México.
Francisco Martínez-Ibáñez,
CENAPA–SENASICA–SADER. Carretera Cuernavaca Cuautla No 8534, Jiutepec, Morelos, C.P. 62550, México.
J. Antonio Álvarez-Martínez,
CENID-Salud Animal e Inocuidad, INIFAP, Carretera Cuernavaca-Cuautla No. 8534, Jiutepec, Morelos, 62550, México.
Carmen Rojas-Martínez,
CENID-Salud Animal e Inocuidad, INIFAP, Carretera Cuernavaca-Cuautla No. 8534, Jiutepec, Morelos, 62550, México.
J. Javier Pérez-de la Rosa,
CENAPA–SENASICA–SADER. Carretera Cuernavaca Cuautla No 8534, Jiutepec, Morelos, C.P. 62550, México.
Grecia Martínez-García,
CENID-Salud Animal e Inocuidad, INIFAP, Carretera Cuernavaca-Cuautla No. 8534, Jiutepec, Morelos, 62550, México.
Rebeca M. Santamaría-Espinosa,
CENID-Salud Animal e Inocuidad, INIFAP, Carretera Cuernavaca-Cuautla No. 8534, Jiutepec, Morelos, 62550, México.
Rebeca M. Santamaría-Espinosa,
CENID-Salud Animal e Inocuidad, INIFAP, Carretera Cuernavaca-Cuautla No. 8534, Jiutepec, Morelos, 62550, México.
Please see the link here: https://stm.bookpi.org/NUAVS-V2/article/view/8919
Keywords: Sheep, Babesia spp, Anaplasma spp, Mycoplasma spp