Molecular Diagnostic Techniques for the Characterisation of Escherichia coli

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Molecular Diagnostic Techniques for the Characterisation of Escherichia coli

January 5, 2021 Biotechnology and Microbiology 0

Escherichia coli isolated from Creek Road/Bonny Estuary using molecular techniques was described by this research. For the presence of E, one hundred and twenty (120) water samples were examined. Coli at Bonny Estuary/Creek Lane. For the isolation and detection of E, the Most Probable Number (MPN) process, the Eijkman test and molecular techniques were used. From coli. The research was performed on a daily basis (morning and evening) and seasonally (end of rainy season (November), beginning of rainy season (April) and mid rainy season (July) respectively). All of E. In the E pathotypes, coli isolates were screened for resistance genes. The Polymerase chain reaction technique uses coli (EHEC, EPEC, EAEC, ETEC and EIEC), including Sulfhydryl Variable (SHV), CTX-M, Temoniera (TEM) and MCR genes. The findings showed that 100% of the water samples were positive for coliforms and that E was harbouring all the water samples. From coli. In the morning of April (48 MPN/100 ml), the highest total count of coliforms recorded was The distribution of in the E of individual genes. SHV (5.0), CTX-M (5.8), TEM (4.2), MCR (0.0 percent), stx1 and stx2 (4.2), esV and bfA (1.7), aaiC (4.2), elt (3.3), and invE are coli isolates: (2.5). Of all the resistance genes, CTX-M was the most frequently detected. The presence of bacteria in the water body may have led to the location of waste dumps, the discharge of sewage and the construction of toilets, and the use of human and animal excreta as manure.

Author(s) Details

Dr. Constancy Prisca Aleru
Department of Medical Laboratory Science, Rivers State University, Nkpolu-Oroworukwo, P.M.B. 5080, Port Harcourt, Rivers State, Nigeria.

Prof. Kinikanwo Confidence Wachukwu
Department of Medical Laboratory Science, Rivers State University, Nkpolu-Oroworukwo, P.M.B. 5080, Port Harcourt, Rivers State, Nigeria.

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