Molecular Identification of Hemoplasmas in Dogs Exposed to Ticks: A Retrospective Study
Canine hemotropic mycoplasmosis (hemoplasmosis) is a affliction guide blood deficiency that has existed stated in dogs accompanying a extensive geographical disposal. The etiologic power is an epierythrocytic microorganisms that is to say situated in the erythrocytes` surface of the touched host. There is a lack of news on hemoplasma contaminations in dogs in Mexico and specifically on the use of microscopic patterns for the discovery concerning this microorganism. This study proposed to act PCR assays and order reasoning to study dogs and ticks for the attendance of dog hemoplasma class.The objective concerning this study search out recognize the occupancy of dog hemoplasmas in archived blood samples composed from 30 dogs and ticks calm from 18 of those dogs. The samples were treated for DNA distillation and elaboration by PCR accompanying 16S rRNA deoxyribonucleic acid primers for Mycoplasma spp. Out of the samples resolved, the PCR assay admitted the labeling of DNA amplicons of the wonted intensity in 20 and 4 of the ancestry and tick samples, respectively. Sequence reasoning utilizing the local adjustment bioinformatics finish (BLASTn) of 8 sequenced samples presented very important correspondence percentages agreeable accompanying the closeness of differing hemoplasma variety in the sequenced samples: Mycoplasma haemocanis (2 samples), Candidatus Mycoplasma haematoparvum (2 samples), Mycoplasma wenyonii (2 samples), Mycoplasma ovis (1 sample), and a nonculturable bacterium (1 sample). It is submitted to complete activity a more painstaking study through the elaboration and sequencing of additional fundamental genes present in the Mycoplasma spp genome, to reinforce either all the recognized hemoplasma variety can efficiently pollute the dog, or the amplified domain of the 16S rRNA deoxyribonucleic acid is not amply changeable between the various hemoplasmas that influence household mammals to admit bias of these Mycoplasma class.
Author(s) Details:
J. Juan Lira-Amaya,
CENID-Salud Animal e Inocuidad, INIFAP, Carretera Cuernavaca-Cuautla No. 8534, Jiutepec, Morelos, 62550, México.
Grecia Martínez-García,
CENID-Salud Animal e Inocuidad, INIFAP, Carretera Cuernavaca-Cuautla No. 8534, Jiutepec, Morelos, 62550, México.
Rebeca M. Santamaría-Espinosa,
CENID-Salud Animal e Inocuidad, INIFAP, Carretera Cuernavaca-Cuautla No. 8534, Jiutepec, Morelos, 62550, México.
Carmen Rojas-Martínez,
CENID-Salud Animal e Inocuidad, INIFAP, Carretera Cuernavaca-Cuautla No. 8534, Jiutepec, Morelos, 62550, México.
J. Antonio Álvarez-Martínez,
CENID-Salud Animal e Inocuidad, INIFAP, Carretera Cuernavaca-Cuautla No. 8534, Jiutepec, Morelos, 62550, México.
J. Javier Pérez-de la Rosa,
CENAPA–SENASICA–SADER, Carretera Cuernavaca Cuautla No 8534, Jiutepec, Morelos, C.P. 62550, México.
Julio V. Figueroa-Millán,
CENID-Salud Animal e Inocuidad, INIFAP, Carretera Cuernavaca-Cuautla No. 8534, Jiutepec, Morelos, 62550, México.
Please see the link here: https://stm.bookpi.org/NUAVS-V2/article/view/8920
Keywords: Tick-infested dogs, Mycoplasma haemocanis, ‘Candidatus Mycoplasma haematoparvum’