Overview of Analytical Methods for Taxanes Quantification in Diluted Formulations and Biological Samples and their Applications in Clinical Practice
The purpose of this study is to examine the analytical methodologies for quantifying taxanes in diluted formulations and biological materials, as well as their clinical applications. Health professionals are currently concerned about improving the rational use of traditional chemotherapy medications, which remain an important method for cancer treatment. Taxanes are a class of chemotherapeutic drugs that are commonly used to treat a wide range of malignancies. Their delivery to patients is parenteral, which entails dilutions from commercial stock solutions in order to acquire the final individualised quantity of medicine prescribed, which is rarely validated. The lack of post-dilution quality control can lead to clinical response variability. The analysis of these diluted solutions is critical for ensuring precise content and, as a result, safe and effective treatment. Therapeutic drug monitoring, on the other hand, is required to optimise individual therapy of medicines having a small therapeutic window, such as taxanes. Drug measurement in plasma samples is critical for establishing pharmacokinetic characteristics that allow dose adjustment and assessing inter- and intra-individual metabolic variability, hence improving therapy efficacy while reducing side effects. As a result, methods for quantifying plasmatic concentrations of taxanes are crucial in clinical practise, helping to treatment individualization. For these goals, this study conducted a bibliographic survey and comparison of currently available analytical methodologies. For quantification of taxanes in hospital laboratories, high performance liquid chromatography (HPLC), liquid chromatography-tandem mass spectrometry (LC-MS/MS), and micellar electro-kinetic chromatography (MEKC) analytical methods were considered and compared. It was concluded that, while LC-MS/MS is an extremely powerful technique, HPLC is versatile and cost-effective for both purposes.
Author (S) Details
Raquel A. Palmas
Requimte/Farma, University of Porto, Faculty of Pharmacy, Department of Drug Sciences, Laboratory of Pharmacology, Porto, Portugal.
Requimte/Farma, University of Porto, Faculty of Pharmacy, Department of Drug Sciences, Laboratory of Pharmacology, Porto, Portugal and Health Sciences Research Center, Department of Pharmaceutical Sciences, Instituto Superior de Ciências da Saúde, Norte, Gandra, Portugal.
Center for Drug Discovery and Innovative Medicines, University of Porto, Porto, Portugal.
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