Preliminary Phytochemical Screening on the Glycosides of Leaf Extracts of Medicinally Important Plant Holoptelea integrifolia (roxb.) Planch Using High Performance Thin Layer Chromatograph
The goal of this work was to employ high-performance thin layer chromatography (HPTLC) to detect glycosides from petroleum ether and methanol extracts of medicinally and commercially beneficial leaves of Holoptelea integrifolia (Roxb.) planch.
Materials and Methods: HPTLC investigations were performed after preliminary phytochemical screening. Linomat V applicator is included with the Camag HPTLC system (Switzerland).
With the help of a tungstant lamp, densitometric scanning was done with a Camag TLC scanner IV in the reflectance absorbance mode at 540 nm using Win computer-aided transcribing system software (1.4.6 Camag).
Results: Steroids, terpenoids, alkaloids, glycosides, flavonoids, proteins, tannins, and carbohydrates were found in preliminary phytochemical screening of H. integrifolia petroleum extract, while steroids, alkaloids, flavonoids, proteins, and carbohydrates were found in preliminary phytochemical screening of H. integrifolia methanolic extract. HPTLC fingerprinting of glycosides from a leaf extract in petroleum ether revealed four polyvalent phytoconstituents (4 peaks) with escalating retention factor (Rf) values in the range of 0.37-0.82. whereas a methanol extract of the leaves revealed ten Polyvalent phytoconstituents (10 peaks) with Rf values in the range of 0.20-0.83 in ascending order.
Conclusions: Based on the Rf values and preliminary phytochemical investigation, we may deduce that both extracts contain glycosides. The plant H. integrifolia was found to be a good source of phytochemicals in this investigation.
Author (s) Details
Ravindra C. Sutar
Department of Pharmacology, Sanjivani College of Pharmaceutical Education and Research, Kopargaon – 423 603, Ahmednagar, Maharashtra, India.
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