Protective and Reversal Effects of N-Acetyl-LCysteine on Moringa oleifera Leaf Extract –Induced Sub-Acute Hepatotoxicity in Wistar Albino Rats

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Protective and Reversal Effects of N-Acetyl-LCysteine on Moringa oleifera Leaf Extract –Induced Sub-Acute Hepatotoxicity in Wistar Albino Rats

November 7, 2020 Medicine and Medical Science 0

Introducing: M. Oleifera is a highly valued medicinal plant that is widely used for the treatment of different ailments. In many tropical & subtropical countries, it is found. The leaves have many medicinal and nutritional values, with the phytochemicals responsible for the medicinal values therein, and when consumed in large quantities, they cause hepatotoxicity. Aim: To decide if N-acetyl cysteine (NAC) has a protective or reversal effect on M. Induced-subacute hepatotoxicity of oleifera leaf aqueous extract in Wistar albino rats. Methods: An experimental laboratory-based study was performed at the University of Makerere, Department of Animal House Physiology. On 48 Wistar Albino rats aged 8-10 weeks, 2 experiments (1) established the protective effects and (2) established the reversal effects of NAC. There were 3 community therapies for the protective effects. The standard saline (1 ml) + toxic dose of M was administered to group I. Group II obtained a toxic dose of acetaminophen (750 mg / kg)+ NAC (50 mg / kg) for oleiferaleaf aqueous extract (8.05 g / kg), and group III received M. (8.05 g / kg), + NAC (50 mg / kg) oleifera. The doses were administered daily for 28 days using an oral intragastric tube. Five treatment groups that produced subacute hepatotoxicity and obtained varying doses of NAC and paracetamol for 7 days had reversal effects. Group 1 received 1 ml of regular saline; Group 2 received 1000 mg / kg of NAC; Group 3 received 1200 mg / kg of NAC; Group 4 received 1,500 mg of NAC; and Group 5 received 1,200 mg / kg of NAC with paracetamol-induced hepatotoxicity. On days 1, 3 and 7, liver enzymes (AST, ALT, and ALP), bilirubin and total serum protein were determined. Both animals were slaughtered under oreuthanized anaesthesia and their liver was extracted for histopathological analysis. Results: Treated animals with a mixture of NAC and a toxic dose of M. On average, oleifera or paracetamol showed normal liver enzymes and levels of bilirubin. NAC-treated animals after M. Hepatotoxicity-induced oleifera and paracetamol displayed normal liver enzymes and levels of bilirubin. The histopathological examination revealed moderate hepatitis characteristics. Conclusion: NAC protections and overturns M. The aqueous extract-induced sub-acute hepatotoxicity of oleifera leaf in Wistar Albino rats.

Author(s) Details

Josephine N. Kasolo
Department of Medical Physiology, Makerere University College of Health Sciences; P.O.Box 7072, Kampala, Uganda.

Agnes Namaganda
Department of Medical Physiology, Makerere University College of Health Sciences; P.O.Box 7072, Kampala, Uganda.

Dr. Godfrey S. Bbosa
Department of Pharmacology and Therapeutics, Makerere University College of Health Sciences; P.O.Box 7072, Kampala,Uganda.

Haruna Muwonge
Department of Medical Physiology, Makerere University College of Health Sciences; P.O.Box 7072, Kampala, Uganda.

Robert Lukande
Department of Pathology, Makerere University College of Health Sciences; P.O.Box7072, Kampala, Uganda.

Dr. Joshua Nfambi
Department of Medical Physiology, Makerere University College of Health Sciences; P.O.Box 7072, Kampala, Uganda.

Ivan Kimuli
Department of Medical Physiology, Makerere University College of Health Sciences; P.O.Box 7072, Kampala, Uganda.

Katamba Godfrey
Department of Medical Physiology, College of Health Sciences, King Caesar University, Kampala, Uganda.

Isaac Okullo
Department of Dental Surgery, Makerere University College of Health Sciences; P.O.Box 7072, Kampala, Uganda.

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