Research on Responses of Digestive and Metabolic Enzymes to Different Feeding Strategies with Sub-optimal Protein Level in Labeo rohita Fingerlings

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Research on Responses of Digestive and Metabolic Enzymes to Different Feeding Strategies with Sub-optimal Protein Level in Labeo rohita Fingerlings

February 17, 2021 Chemistry and Biochemistry 0

In order to research the impact of different feeding strategies with normal and low protein diets on digestive and metabolic function in Labeo rohita fingerlings, a 60-day feeding trial was performed. The most important Indian Major Carp (IMC), which is cultivated commercially across the region, is Labeo rohita. One hundred and thirty-five fingerlings were allocated for each procedure in triplicate groups. Using locally available feed ingredients, three experimental isocaloric (401.32 to 410.28 kcal/100 g) diets of 30 percent, 25 percent and 20 percent crude protein classified as diet A, diet B and diet C respectively were prepared. Three separate feeding schedules of the standard protein diet continuously during the experiment (diet A-30 percent), alternate feeding of 1-day diet A followed by 1-day diet B (1A/1B) and alternate feeding of 1-day diet A followed by 1-day diet C (1A/1C) throughout the experiment were checked by feeding the fish at 5 percent body weight daily. Results showed that in the community fed 1A/1B, digestive enzyme activity such as protease and amylase was greater. The phosphatase of glucose 6 (G6Pase) was also analysed. There is a substantial difference (p<0.05) between the metabolic enzymes such as lactate dehydrogenase (LDH), malate dehydrogenase (MDH) and the various feeding schedules. In the community fed with the 1A/1B feeding schedule, proteolytic activity such as alanine amino transaminase (ALT), aspartate amino transaminase (AST), alkaline phosphatase (ALP) was documented to be greater.

Author (s) Details

Dr. Pankaj Kumar
Department of Fish Nutrition, Biochemistry and Physiology, Central Institute of Fisheries Education, Versova, Mumbai- 400 061, India.

K. K. Jain
Department of Fish Nutrition, Biochemistry and Physiology, Central Institute of Fisheries Education, Versova, Mumbai- 400 061, India.

S. Munilkumar
Department of Fish Nutrition, Biochemistry and Physiology, Central Institute of Fisheries Education, Versova, Mumbai- 400 061, India.

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