Screening Methods for the Detection of Antagonistic Actinobacteria: Facts and Problems

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Screening Methods for the Detection of Antagonistic Actinobacteria: Facts and Problems

August 11, 2020 Biotechnology and Microbiology 0

Screening is the highly selective procedures for the detection and isolation of only those
microorganisms of interest from among a large microbial population. Actinobacteria are the group of
bacteria with the greatest biomedical value especially antibiotic production. Detection of the
antagonistic property of actinobacteria is the pre-requisite for their exploitation for antibiotic
production. There are several methods described as well as in practice for the detection of
antagonistic actinobacteria. All the screening methods are broadly categorized into diffusion methods
(crowded plate, agar overlay, cross streak, cross spot, drop test, agar plug) and dilution (agar dilution,
broth dilution) methods. Some important methods commonly used for the detection of antagonistic
activity of actinobacteria and their advantages and limitations are described in this chapter.

Author(s) Details

Dr. Manikkam Radhakrishnan
Centre for Drug Discovery and Development, Sathyabama Institute of Science and Technology, Chennai – 600 119, Tamil Nadu, India.

Mr. Kaari Manigundan
Centre for Drug Discovery and Development, Sathyabama Institute of Science and Technology, Chennai – 600 119, Tamil Nadu, India.

Dr. Venugopal Gopikrishnan
Centre for Drug Discovery and Development, Sathyabama Institute of Science and Technology, Chennai – 600 119, Tamil Nadu, India.

Dr. Ramasamy Balagurunathan
Department of Microbiology, Periyar University, Salem 636 011, Tamil Nadu, India

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