Screening of Punica granatum Seeds for Antibacterial and Antioxidant Activity with Various Extracts: A Brief Analytical Study

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Screening of Punica granatum Seeds for Antibacterial and Antioxidant Activity with Various Extracts: A Brief Analytical Study

July 1, 2021 Chemistry and Biochemistry 0

Punica granatum thrives in climates with long, hot, dry summers (90°F) and milder winters. The genus name is derived from the Latin name Punicum malum. A 100-g serving of pomegranate seeds contains 12% of the Daily Value (DV) for vitamin C, 16% of the DV for vitamin K, and 10% of the DV for folate. Pomegranate seeds are high in dietary fiber (20% DV), which is completely contained in the edible seeds. People who choose to throw away the seeds miss out on the nutritional benefits provided by seed fiber and micronutrients [1]. The most abundant phytochemicals in pomegranate are the hydrolyzable tannins known as ellagitannins, which are formed when ellagic acid and/or gallic acid bind with a carbohydrate to form pomegranate ellagitannins [2,3], also known as punicalagins Anthocyanins are responsible for the red color of juice. Generally, as fruit ripens, the pigmentation of the juice increases [4]. Processing and pasteurization techniques reduce the phenolic content of pomegranate juice [5]. The antimicrobial activity of the various extracts (maceration with ethanol and hexane separately, and soxhlet with ethanol) was investigated (using agar well diffusion method). Positive controls included penicillin and ampicillin. Ethanol soxhlet extracts inhibited the growth of all seven strains of bacteria, resulting in the detection of a zone of inhibition. Ethanol maceration extract only had antimicrobial activity against Neisseria gonorrhoeae, Pseudomonas aeruginosa, and Staphylococcus aureus, whereas hexane extract did not. Using the equations obtained from the graphs, the IC50, or half maximal (50 percent ) inhibitory concentration for Punica granatum seed extracts, was calculated. The DPPH radical scavenging assay was used to determine antioxidant activity. In this study, the half maximal (50 percent) inhibitory concentration (IC50) was determined. Ethanol soxhlet (ES) extract had the lowest IC50 value (1350.27 g/mL), hexane extract (HM) had the highest IC50 value (3005.66 g/mL), and ethanol maceration extract (EM) had the lowest. (4852.26 g/mL) value The following objectives were devised to achieve the goals: Authentication of plant material The antimicrobial potential and antioxidant activity of Punica granatum seed extracts were evaluated.

Author(s) Details

Naeem Hasan Khan
Faculty of Pharmacy, AIMST University, Malaysia.

Adriana Lee Tze Ying
Faculty of Pharmacy, AIMST University, Malaysia.

Candy Goo Zhi Tian
Faculty of Pharmacy, AIMST University, Malaysia.

Ooi Wei Yi
Faculty of Pharmacy, AIMST University, Malaysia.

Shantini Vijayabalan
Faculty of Pharmacy, AIMST University, Malaysia.

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