Study on Increased Fructosyltranseferase (levansucrase) Production by Optimizing Culture Condition from Pediococcus acidilactici Strain in Shaking Batch Cultures

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Study on Increased Fructosyltranseferase (levansucrase) Production by Optimizing Culture Condition from Pediococcus acidilactici Strain in Shaking Batch Cultures

November 12, 2021 BIOLOGY 0

Polymerases involved in microbial fructan (levan, inulin, and fructo-oligosaccharide) production are known as microbial fructosyltransferases (levansucrases). Levan could be used in the culinary, pharmaceutical, medical, cosmetics, and textile industries. Microbial fructosyltransferase proteins are classified into seven phylogenetically related clusters that share the catalytic domain of the glycoside hydrolases 68 family. The goal of the study was to see how effective some bacterial isolates were at producing extracellular levansucrase. Bacteria were isolated from a variety of places (oral swab, spoiled milk, spoiled whey, and spoiled yoghurt). After 2 days of fermentation under shacking conditions, Pediococcus acidilactici had the highest levansucrase activity (12.64 U/ml) and the highest levan output (15.4 g/L). In submerged liquid cultures, the carbon source, nitrogen source, temperature, and starting pH of the growth media are all optimised. Sucrose (30% w/v) was shown to be the most effective inducer of levansucrase formation. The temperature and pH of the levansucrase activity were found to be optimum at 30°C and 6.0, respectively. Pediococcus acidilactici extract crude levansucrase was enriched using a variety of precipitating agents. The results demonstrate that ethanol (65% saturation) offered the maximum purification factor of 3-fold, with a total highest recovered protein recovery yield of 41.05 percent. Semi purified levansucrase’s optimal reaction temperature and pH were 40°C and 5.2, respectively. SDS–PAGE analysis of the partially purified enzyme preparation revealed one levansucrase-active protein band. This band’s molecular weight is estimated to be around 50.000 Daltons. The findings imply that Pediococcus acidilactici levansucrase is quite efficient at converting sucrose to levan, making it potentially useful as a food additive and in medicine as a plasma substitute, medication activity prolongator, and antihyperlipidemic agent with anti-diabetic properties.

Author(S) Details

Ghada A. Youssef
Botany and Microbiology Department, Faculty of Science, Alexandria University, Egypt.

Amany S. Youssef
Botany and Microbiology Department, Faculty of Science, Alexandria University, Egypt.

Shimaa Talha
Botany and Microbiology Department, Faculty of Science, Alexandria University, Egypt.

Samy A. El-Aassar
Botany and Microbiology Department, Faculty of Science, Alexandria University, Egypt.

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