Assessment and Analyses of Homing Endonucleases and Mechanism of Action of CRISPR-Cas9 HNH Endonucleases
Objective: To study various HNH endonucleases from different sources, including CRISPR-Cas9 protein HNH endonuclease regions, for their conserved motifs, metal binding sites and catalytic amino acids, and to propose a plausible mechanism of action for HNH endonucleases using CRISPR-Cas9 Streptococcus pyogenes as the model enzyme. Design of the study: Multiple sequence analysis (MSA) was studied…
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